同調反史托克拉曼散射系統CARS

System Introduction:

♦ High sensitivity: CARS generates more intensive and directed signals in comparison to       spontaneous Raman microscopy.

♦ Anti-Stokes CARS signal has frequency exceeding pumping waves frequencies and is           detected in a spectral range free from the stray light of Stokes luminescence.

♦ CARS signal is registrated only in a focus where excitation intensity is the highest. It           allows imaging with a high spatial resolution using non-confocal pinholes and also               performs 3D layer-by-layer scanning with minimal neighboring layers influence on               measuring results.

♦ Spectral resolution of CARS signal is defined only by the width of pumping laser lines,         what simplifies spectral measurements, as detection of CARS signals can be performed     without any spectral instrument.

♦ CARS signal is proportional to the squared molecule concentration, it allows using CARS     for quantitative measurements of chemical substance concentration in a sample;

♦ Minimal invasive (nondestructive) CARS method for biological samples. Due to the high     sensitivity of CARS method molecules in living cells can be detected without                           fluorescent   markers.

Multimodal CARS/TPEF image of growing cancer cells HeLa: DNA/RNA/Proteins/Lipids colored